The Flying Eagles will battle Nasarawa United in a test game in Abuja tomorrow.
The friendly will be played at the FIFA Goal Project Abuja.
Kick-off time is 3.30pm.
The Nigeria U20s played out a 0-0 draw with another NPFL club Katsina United in Katsina on Wednesday.
Coach Paul Aigbogun said the recent matches played by the Flying Eagles have given him a better idea of the team he will line up against Guinea Bissau.
The Nigeria U20s are preparing for a 2019 U20 AFCON qualifier against Guinea Bissau to be played in Bissau between May 11 and 13.
The return leg will be played in Nigeria a week later.
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National Team